Platform for Drug Discovery



Bowtie(PE)-MACS2-GADEM-MotIV-ChIPpeakAnno


Introduction


    This pipeline predict protein binding regions by MACS2 which work well for sharp peak.


    Input1 formatFASTQ(ChIP/case)
    Input2 formatFASTQ(Input/control)
    Library layoutPaired-end
    SpeciesHuman
    Execution timeAbout 6 hours. (29M and 21M reads paired-end [75bp+75bp])

Table of contents

Inputs


    Raw NGS reads (FASTQ format, paired-end): 2


Outputs


    Workflow
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    HTML Report
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    (1) ChIP-seq QC report
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    (2) Enriched regions (BED format)
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    (3) Enriched region (Genome Explorer)


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    (4) Peak call summary report
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    (5) De novo motif report
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    (6) Known motif report
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    (7) TSS and GO annotation report

Options


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    (partially omitted)

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